Please tell me, should I use the comma in this case? Thank you!
Currently , the most spread pathogenic organisms in strawberry plantations and strawberry crop rotations under conditions of our region are 1)..,2)..,3)...
Yes and no. Inserting a comma after a short or one-word introductory has become a style choice. Many publishers--at least, in the USA--no longer use a comma for an introductory phrase with fewer than three words.
However, I would keep it there.