Yes. (It was considered bad form to end a sentence with a preposition, but people do it all the time, because in many contexts it sounds so much more natural). E.g.:
Which department are you in charge of?
These are the projects that I'm in charge of.
There are alternative ways of saying such sentences that avoid the preposition at the end -- Which department do you manage? These are the projects that I'm overseeing. -- but most people would find a sentence ending with "...in charge of" perfectly natural.