You can't always eliminate articles (in thesis topics), but I'd go with "..in Spanish noir comics." You could say, for instance, "...in the Spanish noir comics of the 1930s." (or "...in Spanish noir comics in the 1930s.")
Hi, m4tt, I think Szkot meant you would use "A Study of Spanish..." at the beginning of your title ('a/an' is the indefinite article; I don't think Szkot meant you should put the definite article 'the' before 'Spanish'). Check what style your advisor prefers for capitaization in thesis titles, too.